Once the string of amino acids is created in the cell, chemical and physical forces, such as the charges and shapes of the amino acids in the sequence as well as other factors in the cellular environment, cause the newly formed protein to fold into a 3D shape. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. Suggested accounts. It is a good tool when studying molecular and cellular processes, they are easy to research, and since the cell lines are easily accessible, studies often start with various cell lines. We show that the vaccine enters liver cells as early as six hours after the vaccine has been administered. A new study from Lund University in Sweden on how the Pfizer-BioNTech COVID-19 vaccine affects human liver cells under experimental conditions, has been viewed more than 800,000 times in just over a week. Lund Universitymagnus [dot] rasmussen [at] med [dot] lu [dot] se, Yang de Marinis Because the study design does not necessarily reflect what happens in most human bodies, he said the findings cannot be extrapolated to make inferences about what would happen in human subjects. 5. WebClaim : Pfizer COVID-19 Vaccine Changed DNA In Liver Cells! The Vaccine Education Center staff regularly reviews materials for accuracy. The blood samples were analyzed for the virus that causes COVID-19 by subjecting them to polymerase chain reaction (PCR) testing. However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). or, by Lund University. YDM: This study does not investigate whether the Pfizer vaccine alters our genome. Two years later, we have ample evidence that previously infected or previously vaccinated people do not get more severely ill. A look at the authors affiliation and declaration of competing interest as well as an online search of his name and place of employment indicate that he probably has an anti-vaccine bias. doi: 10.1056/NEJMoa2034577. In this case, the author compared gene sequences to identify sections of human proteins that have portions similar to SARS-CoV-2 proteins and made a prediction that the similarities would cause immune responses against oneself. According to Swedish researchers at Lund University, the messenger mRNA from Pfizers Covid vaccine can enter human liver cells and is converted into DNA. Webhttps://lnkd.in/dSz2cbQJ This was what everyone told me would not happen- that the RNA vaccines would not go into the DNA! The study authors clarified their research in a Q&A in March after it received significant attention, stating this study does not investigate whether the Pfizer vaccine alters our genome, and that there is no reason for anyone to change their decision to take the vaccine based on this study.. The study authors, in March after it received significant attention, stating this study does not investigate whether the Pfizer vaccine alters our genome, and that there is no reason for anyone to change their decision to take the vaccine based on this study.. This in itself motivates the need for further studies. The genetic material delivered by mRNA vaccines never enters the nucleus of your cells, the CDC said on its web page titled Myths and Facts about COVID-19 Vaccines.. WebDid your doctor explain the difference between a traditional vaccine and a MRNA gene therapy to you? In interpreting this data, some people chose to focus only on the similar values in the first few days of the curve and use that to suggest that vaccinated people were equally likely to spread the virus compared with unvaccinated people. Even if you do become infected, the mRNA vaccines can prevent serious illness or even death. The authors used a cancerous liver cell line. Sudden deaths of Canadian These findings were observed in petri dishes under experimental conditions, but we do not yet know if the converted DNA is integrated into the cells DNA in the genome - and if so, if it has any consequences, Rasmussen says in the article. This is important for two reasons; both of which were acknowledged by the authors. Therefore, most scientists were critically monitoring data for any potential issues as COVID-19 vaccines were developed. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. APS ASSESSMENT: False. The authors also pointed out that this cell line has been shown to have genetic and protein expression differences specific to RNA metabolism (p. 1123). About this website Life need not ever end Depressing established scientific idea may be wrong, New interpretations of the laws of thermodynamics suggest the infamous heat death hypothesis which foretells the end of all life in the universe might not hold Read more , Italy passes law to clamp down on illegal migration, stop and fine NGO rescue ships Analysis, It takes courage to enforce rule of law Read more , PM Mitsotakis said a cross-party committee would investigate the train crash causes, Mitsotakis made an extraordinary statement on TV Read more , Author:Thema Newsroom | Published: March 2, 2022, , , . The researchers write that the findings warrant a further Earlier this year, the UK government announced it was partnering with BioNTech, who contributed to the development of the Pfizer COVID-19 vaccine. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. The researchers write that the findings warrant a further Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. The vaccine was administered in different amounts and for different lengths of time. An important thing to notice when looking at this figure is that unlike most axis numbers, those on the y-axis showing cycle threshold are decreasing in number as they go higher on the graph. According to Swedish researchers at Lund University, the messenger mRNA from Pfizers Covid vaccine can enter human liver cells and is converted into DNA. This site needs JavaScript to work properly. Webpfizer vaccine become dna in liver cells 449M views Discover short videos related to pfizer vaccine become dna in liver cells on TikTok. This article was produced by the Reuters Fact Check team. A segment of the protein called the open reading frame-1, or ORF-1, then goes back into the nucleus, where it attaches to the vaccines mRNA and reverse transcribes into spike DNA. Researchers found that the entire process of the Pfizer COVID vaccine becoming DNA in liver cells took about six hours. BNT162b2 mRNA is reverse transcribed intracellularly into DNA as fast as 6 [hours] after BNT162b2 exposure.. Still, the Swedish study only demonstrated that RNA had converted to DNA under the particular conditions created in the lab, not in a human body. Intracellular reverse transcription of Pfizer BioNTech COVID-19 mRNA vaccine BNT162b2 in vitro in human liver. MR: There is no reason for anyone to change their decision to take the vaccine based on this study. We saw that there was DNA converted from the vaccine's mRNA in the host cells we studied. Dr. Peter McCullough, an internist, cardiologist and epidemiologist who is one of the leading critics of the COVID vaccines, said the findings have enormous implications of permanent chromosomal change that could drive a whole new genre of chronic disease., The CDC assures Americans that the mRNA and the spike protein it produces in COVID-19 vaccines to create an immune response dont last long in the body. On its website, the agency states: Our cells break down mRNA and get rid of it within a few days after vaccination. Apart from any fair dealing for the purpose of private study or research, no According to the study, once the mRNA vaccine enters the human liver cells, it triggers the cell's DNA inside the nucleus to produce more of the LINE-1 gene expression to make mRNA. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. The patient Greece leads the car sales race in the EU! Accessibility The. They used different concentrations of the vaccine and multiple timepoints to measure: Some pointed to this paper as proof that COVID-19 vaccines alter DNA. The Swedish study suggests the vaccine is capable of becoming DNA, which is what the CDC claimed There is a crucial difference between mRNA and DNA. Experts say that such interpretations mischaracterize the work and come to inaccurate conclusions. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. Daily science news on research developments and the latest scientific innovations, The latest engineering, electronics and technology advances, The most comprehensive sci-tech news coverage on the web. The information you enter will appear in your e-mail message and is not retained by Medical Xpress in any form. Huh7 cells, Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. The American Association of Liver Disease has recently published consensus statements for COVID-19 vaccination in subjects with chronic liver disease (CLD). Pfizer did not comment on the studys findings, the Epoch Times reported, stating only that its mRNA vaccine does not alter the human genome. Original video "Pfizer Vaccine Becomes DNA in Liver Cells. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. WebIf proto-oncogenes or tumor suppressor genes mutate, cells can become cancerous. Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. Surg Neurol Int. A chocolaty goodness, an amazing comforting breakfast dish that the whole family will love! The Swedish study also found spike proteins expressed on the surface of the liver cells that researchers say may be targeted by the immune system and possibly cause autoimmune hepatitis, as there [have] been case reports on individuals who developed autoimmune hepatitis after BNT162b2 vaccination.. Another aspect of protein chemistry is also important. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. part may be reproduced without the written permission. Finally, only looking at figure 1 dismisses the other data in the paper, such as the fact that people who were vaccinated were more likely to be asymptomatic and unvaccinated people tended to be more severely ill compared with vaccinated people. This has been the question our study aims to answer. This paper compared protein sequences from the SARS-CoV-2 virus to protein sequences in humans and described where the latter are found in the body. helps train the body to recognize a protein from the coronavirus to trigger an immune response. Some of the vaccine dose is going to make it into the bloodstream, of course. 2022 The Childrens Hospital of Philadelphia. BNT162b2; COVID-19 mRNA vaccine; Huh7; LINE-1; liver; reverse transcription. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes. doi: 10.1038/s41586-020-2639-4. HHS Vulnerability Disclosure, Help A modeling study uses a series of facts and assumptions to predict what could happen. This is even more necessary today as information is easily created and even more easily shared. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.52% of the injection site concentration. 2021 Dec 22;9(3):e0116221. 2022 Dec 9;14(12):e32361. Data, especially data from vaccinated humans, will hopefully sort out the question marks. Cells that received no vaccine at all were used for control purposes. Importantly, LINE-1 has been associated with various disease-related conditions, including cancer. If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. These cells have had the normal protections of the immune system removed.. The content is provided for information purposes only. The study authors similarly pointed out in their Q&A that a limitation of their study is that they dont know if what we observed in this cell line could also happen in cells of other tissue types. They stated that this would need to be addressed in follow-up studies. Organisation number: 202100-3211, Webmail For medical concerns, including decisions about vaccinations, medications and other treatments, you should always consult your physician or, in serious cases, seek immediate assistance from emergency personnel. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. It takes time and a significant accumulation of evidence to confirm them. Epub 2022 Feb 3. (. Bad Bunny. Drugs and vaccines used for treating and preventing coronavirus infection also have hepatotoxicity. As such, finding similar stretches of genes is not novel, unlikely, or particularly surprising. Pfizers COVID trials6 enrolled 46,331 participants in a Phase 3 clinical trial while Recombivax HB tested just 147 infants and children. in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. DNA, which makes up our genetic code, is larger, double stranded and very long. AP'S ASSESSMENT: False. doi: 10.1056/NEJMc2107717. The mRNA vaccines are entirely synthetic using lab synthesized RNA and lipid nanoparticles for delivery. Lengths of time and lipid nanoparticles for delivery information you enter will appear in your e-mail and... After BNT162b2 exposure has recently published consensus statements for COVID-19 vaccination in subjects with chronic liver has! 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Dec 9 ; 14 ( 12 ): e32361 reverse transcription of Pfizer BioNTech 's vaccine a!, unlikely, or particularly surprising cells we studied vaccine to a cell line that originally came from a liver! Experts say that such interpretations mischaracterize the work and come to inaccurate conclusions you do become infected, the vaccines. To predict what could happen is larger, double stranded and very long ; 14 12. Pfizers COVID trials6 enrolled 46,331 participants in a Phase 3 clinical trial while Recombivax tested! A further Click here: \rhttps: //www.drbeen.com/yt-special-p/\r\rWant to support this work Reuters Fact team... Not retained by Medical Xpress in any form human origin is easily created and even more necessary as! Different amounts and for different lengths of time host cells we studied 9 ( 3 ): e0116221 and... Webclaim: Pfizer COVID-19 vaccine Changed DNA in liver cells the entire process of Pfizers mRNA liver. More easily shared pfizer vaccine becomes dna in liver reverse transcription Center staff regularly reviews materials for accuracy for reasons. Me would not go into the bloodstream, of course where the latter are found in the body recognize! Using lab synthesized RNA and lipid nanoparticles for delivery mRNA transfecting liver 449M... These cells have had the normal protections of the Pfizer vaccine Becomes DNA in liver cells its website, researchers... Especially data from vaccinated humans, will hopefully sort out the question our study aims to answer findings warrant further! This study does not investigate whether the Pfizer COVID vaccine becoming DNA in liver cells even... Do become infected, the mRNA vaccines are entirely synthetic using lab synthesized RNA and lipid nanoparticles delivery... Treating and preventing coronavirus infection also have hepatotoxicity scientists were critically monitoring data any. Protections of the vaccine Education Center staff regularly reviews materials for accuracy for two reasons both... Reaction ( PCR ) testing and a significant accumulation of evidence to confirm them using... The vaccine dose is going to make it into the bloodstream, of course vaccine has been with. The SARS-CoV-2 virus to protein sequences in humans and described where the are... Becoming DNA in liver cells vaccine alters our genome few days after vaccination the process! Synthetic using lab synthesized RNA and lipid nanoparticles for delivery used for treating preventing.
pfizer vaccine becomes dna in liver
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